| 1. | Therefore , blys , its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity , neoplasia , or immunodeficiency syndromes . in this study , epo signal peptide sequence and hsblys gene were linked by soe method . the fusion product was cloned into eukaryotic plasmids . pcdna3 , pcdna3 . 1 , pefneo , respectively . meanwhile , the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i . thus the recombinant plasmid can be used as secreting plasmid expressing other gene
本实验通过3 ’端互补，进行引物延伸合成epo信号肽序列：信号肽和hsblys基因采用重叠延伸拼接法形成融合基因；融合基因分别插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核载体：引物延伸合成信号肽时，利用亮氨酸同义密码，将信号肽基因的倒数第二个密码突变，在重组载体上的信号肽序列之后，形成bln酶切位点，使三种载体成为分泌表达载体。 |
